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crispr cas9 expression vector pspcas9 bb 2 a puro  (Addgene inc)


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    Structured Review

    Addgene inc crispr cas9 expression vector pspcas9 bb 2 a puro
    Crispr Cas9 Expression Vector Pspcas9 Bb 2 A Puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 2962 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/crispr+cas9+expression+vector+px459/pSpCas9(BB)-2A-Puro+(PX459)+V2%2E0+(Plasmid+%2362988)/pm41330077-61-1-7
    Average 96 stars, based on 2962 article reviews
    crispr cas9 expression vector pspcas9 bb 2 a puro - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Clone Assay:

    Article Title: RTN4IP1 is essential for the final stages of mitochondrial complex I assembly and coenzyme Q biosynthesis
    Article Snippet: .. The RTN4IP1 sgRNA was cloned into CRISPR-Cas9 expression vector px459 (Addgene). ..

    Article Title: RTN4IP1 is required for the final stages of mitochondrial complex I assembly and CoQ biosynthesis
    Article Snippet: .. The RTN4IP1 sgRNA was cloned into the CRISPR-Cas9 expression vector px459 (Addgene). ..

    Article Title: RTN4IP1 is required for the final stages of mitochondrial complex I assembly and CoQ biosynthesis
    Article Snippet: .. The RTN4IP1 sgRNA was cloned into the CRISPR-Cas9 expression vector px459 (Addgene). ..

    CRISPR:

    Article Title: RTN4IP1 is essential for the final stages of mitochondrial complex I assembly and coenzyme Q biosynthesis
    Article Snippet: .. The RTN4IP1 sgRNA was cloned into CRISPR-Cas9 expression vector px459 (Addgene). ..

    Article Title: RTN4IP1 is required for the final stages of mitochondrial complex I assembly and CoQ biosynthesis
    Article Snippet: .. The RTN4IP1 sgRNA was cloned into the CRISPR-Cas9 expression vector px459 (Addgene). ..

    Article Title: RTN4IP1 is required for the final stages of mitochondrial complex I assembly and CoQ biosynthesis
    Article Snippet: .. The RTN4IP1 sgRNA was cloned into the CRISPR-Cas9 expression vector px459 (Addgene). ..

    Expressing:

    Article Title: RTN4IP1 is essential for the final stages of mitochondrial complex I assembly and coenzyme Q biosynthesis
    Article Snippet: .. The RTN4IP1 sgRNA was cloned into CRISPR-Cas9 expression vector px459 (Addgene). ..

    Article Title: RTN4IP1 is required for the final stages of mitochondrial complex I assembly and CoQ biosynthesis
    Article Snippet: .. The RTN4IP1 sgRNA was cloned into the CRISPR-Cas9 expression vector px459 (Addgene). ..

    Article Title: RTN4IP1 is required for the final stages of mitochondrial complex I assembly and CoQ biosynthesis
    Article Snippet: .. The RTN4IP1 sgRNA was cloned into the CRISPR-Cas9 expression vector px459 (Addgene). ..

    Plasmid Preparation:

    Article Title: RTN4IP1 is essential for the final stages of mitochondrial complex I assembly and coenzyme Q biosynthesis
    Article Snippet: .. The RTN4IP1 sgRNA was cloned into CRISPR-Cas9 expression vector px459 (Addgene). ..

    Article Title: RTN4IP1 is required for the final stages of mitochondrial complex I assembly and CoQ biosynthesis
    Article Snippet: .. The RTN4IP1 sgRNA was cloned into the CRISPR-Cas9 expression vector px459 (Addgene). ..

    Article Title: RTN4IP1 is required for the final stages of mitochondrial complex I assembly and CoQ biosynthesis
    Article Snippet: .. The RTN4IP1 sgRNA was cloned into the CRISPR-Cas9 expression vector px459 (Addgene). ..



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    Addgene inc crispr cas9 expression vector plasmid px459
    Deletion of the Runt domain in A375 melanoma cells. ( A ) PCR products. 11 clones have been generated by applying the <t>CRISPR/Cas9</t> technique. The PCR products, analysed by agarose gel electrophoresis, have been obtained by amplifying the Runt domain region. ( B ) Sanger sequencing chromatogram. The image shows the comparison between the WT and the 3G8 sequences with the deletion in genomic context.
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    Addgene inc crispr cas9 expression vector pspcas9 bb 2a puro px459
    Deletion of the Runt domain in A375 melanoma cells. ( A ) PCR products. 11 clones have been generated by applying the <t>CRISPR/Cas9</t> technique. The PCR products, analysed by agarose gel electrophoresis, have been obtained by amplifying the Runt domain region. ( B ) Sanger sequencing chromatogram. The image shows the comparison between the WT and the 3G8 sequences with the deletion in genomic context.
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    Image Search Results


    Deletion of the Runt domain in A375 melanoma cells. ( A ) PCR products. 11 clones have been generated by applying the CRISPR/Cas9 technique. The PCR products, analysed by agarose gel electrophoresis, have been obtained by amplifying the Runt domain region. ( B ) Sanger sequencing chromatogram. The image shows the comparison between the WT and the 3G8 sequences with the deletion in genomic context.

    Journal: Scientific Reports

    Article Title: Runx2 stimulates neoangiogenesis through the Runt domain in melanoma

    doi: 10.1038/s41598-019-44552-1

    Figure Lengend Snippet: Deletion of the Runt domain in A375 melanoma cells. ( A ) PCR products. 11 clones have been generated by applying the CRISPR/Cas9 technique. The PCR products, analysed by agarose gel electrophoresis, have been obtained by amplifying the Runt domain region. ( B ) Sanger sequencing chromatogram. The image shows the comparison between the WT and the 3G8 sequences with the deletion in genomic context.

    Article Snippet: The above gRNAs were individually cloned in the PX459 V2.0 Cas9 expressing vector (Addgene), following the protocol described by Ran et al . .

    Techniques: Clone Assay, Generated, CRISPR, Agarose Gel Electrophoresis, Sequencing, Comparison